SPRi measurements

The SPRi measurements were performed in a temperature‐controlled HORIBA Scientific SPRi‐PLEX II apparatus. Briefly, Icaritin was added onto the photo‐crosslingker chip and was irradiated with UV photocrosslinker chamber at 365 nm wavelength for 15 min. After washed with enthanol and water, protein lysate from 1 × 10⁷ SMMC‐7721 cells was loaded onto the chip and washed with PBS to remove unbound proteins. Then the bound proteins were eluted with 1% SDS and reloaded onto the chip for a second round screening. After two rounds screening, the eluted samples were subject to LC‐MS profiling.