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UV‐induced premutagenic DNA lesions in a 3‐D human skin model

MB Manuela Buonanno
DW David Welch
DB David J. Brenner
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We used the 3‐dimensional (3‐D) human skin model EpiDerm‐FT (MatTek Corp., Ashland, MA) consisting of stratum corneum and 8‐12 human cell layers to reproduce human epidermis and dermis (24). We measured induction of the two most abundant premutagenic DNA lesions, cyclobutane pyrimidine dimers (CPD) and pyrimidine‐pyrimidone 6‐4 photoproducts (6‐4PP), in keratinocytes of the epidermis (25) as a function of the UVC radiant exposure. Sham and exposed tissues were fixed 15 min after exposure, and the DNA photoproducts were detected using the immunohistochemical method previously described (26).

Copyright and License information:  ©2021 The Authors. published by Wiley Periodicals LLC on behalf of American Society for Photobiology.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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