Genomic DNA was extracted using boiling-freezing method. Briefly, suspension of each isolate in sterile deionized water was boiled for 10 min and immediately placed on ice for 5 min. After three cycles of freezing-thawing, the tubes were centrifuged. The supernatants were removed and stored at − 20 °C as DNA template until experiments. The quantity of each DNA extract was determined by measuring the absorbance at 260 nm to estimate DNA concentration and by calculating the ratio of A260/A280 to determine purity. The DNA was considered to be pure when the A260/A280 ratio was within the range of 1.6–2.0 [18].
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