FST and OFT

The FST was executed similarly to that described previously (Neckameyer and Nieto-Romero, 2015). For the learning phase, male flies were placed for 5 min in a round plastic well (diameter 3.2 cm, depth 1 cm) filled with 3 ml water/0.0025% Triton X-100 solution. Afterwards, the male fly was dried on a piece of tissue and placed back in a vial overnight together with a female at 25°C. 24 h later, the male fly was put back on the water/0.0025% Triton X-100 solution and filmed for 5 min. Survivors of this procedure who were able to walk in a dry vial afterwards were included in the analysis. For further data analysis, the immobile time was counted from the video.

Details on the OFT and other methods used to collect the data in Figs S1-S6 and Table S1 are provided in the Supplementary Materials and Methods.