ROA and Raman spectroscopy

ROA and Raman spectra were recorded simultaneously on a BioTools ChiralRAMAN© spectrometer (BioTools Inc, Jupiter FL, USA). ROA activity was measured as the intensity difference between the scattered radiations right and left circularly polarized (SCP). Spectra were recorded at the room temperature from 100 μl of saturated solutions in deuterated dichloromethane, CD₂Cl₂. Excitation wavelength at 532 nm was used as generated by a Nd:VO₄ laser working at a power below 20 mw. Backscattering collection of the scattered radiation was used with a spectral resolution of 7 cm⁻¹ and acquisition times of about 24 h. For each spectrum, at least two different experiments were carried out from independently prepared samples.