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Protein Identification by Mass Spectrometry
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The proteins pulled down by streptavidin magnetic beads were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel. Then gel was stained using a protein silver stain kit (CWBio, Beijing, China) according to the manufacturer’s protocols. The target protein bands were excised from the gel and subjected to MS. Beijing Protein Innovation Co., Ltd. (Beijing, China) performed the MS assay.
Copyright and License information: ©2021 Li, Zhang, Li, Guo, Li, Zhang, Li and Mi.
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