Calibrated automated thrombography was used to determine endogenous thrombin generation in murine PPP according to the method described by Tchaikovski et al with slight modifications [19, 23].
The ex vivo effects of dapagliflozin in murine and human PPP were tested after preincubation with dapagliflozin (0.5 μmol/l, Sigma-Aldrich) for 30 min at 37°C with HDL-cholesterol (5.2 mmol/l, Sigma-Aldrich) using PPP LOW-reagent, Thrombin Calibrator and FluCa-Kit (Diagnostics Stago). The respective buffer without HDL was used as control sample.
Electronic supplementary material (ESM) Table 2 shows the platelet characteristics.
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