Calibrated automated thrombography

CK Christina Kohlmorgen
SG Stephen Gerfer
KF Kathrin Feldmann
ST Sören Twarock
SH Sonja Hartwig
SL Stefan Lehr
MK Meike Klier
IK Irena Krüger
CH Carolin Helten
PK Petra Keul
SK Sabine Kahl
AP Amin Polzin
ME Margitta Elvers
UF Ulrich Flögel
MK Malte Kelm
BL Bodo Levkau
MR Michael Roden
JF Jens W. Fischer
MG Maria Grandoch
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Calibrated automated thrombography was used to determine endogenous thrombin generation in murine PPP according to the method described by Tchaikovski et al with slight modifications [19, 23].

The ex vivo effects of dapagliflozin in murine and human PPP were tested after preincubation with dapagliflozin (0.5 μmol/l, Sigma-Aldrich) for 30 min at 37°C with HDL-cholesterol (5.2 mmol/l, Sigma-Aldrich) using PPP LOW-reagent, Thrombin Calibrator and FluCa-Kit (Diagnostics Stago). The respective buffer without HDL was used as control sample.

Electronic supplementary material (ESM) Table 2 shows the platelet characteristics.

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