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Nematodes were grown, maintained, and manipulated with standard techniques [35, 41]. We utilized the C. elegans strains N2 (wild type), AU37 [glp-4(bn2) I; sek-1(km4) X], and SS104 [glp-4(bn2) I]. Worm strains were provided by the Caenorhabditis Genetic Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Synchronized C. elegans cultures were obtained using standard protocols [35, 41]
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