Mice were acclimatized to the testing room and trained by placing them on an accelerating rotarod for a maximum of 60 s at low speed, three times with training taking place on two consecutive days. On testing days (48 hr apart), mice were injected with CNO or saline 30 min before being placed on the rotarod. Latency to fall was measured for up to 300 s. The procedure was repeated three times and latencies averaged across trials.
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