2.4. Preparation of Plant Extract

The plant parts were collected, cleaned, and converted to a fine powder after proper shade drying. A double cold maceration extraction procedure was performed by taking 200 g leaves of different regions, soaked with 1000 mL of ethyl acetate and ethanol in different conical flasks with occasional shaking for 72 hours. Liquids were strained and filtered. The process was repeated up to double maceration and the filtrate was mixed and then dried by using a rotary vacuum evaporator at a temperature of 40°C. The extract was placed in glass vials and extractive yields were determined. All the airtight vials were preserved in the refrigerator at 4°C until use.