Histamine H2R agonist (dimaprit) administration

Mice were inoculated with P. yoelii–infected or control blood as previously described. In the morning of day 6 after infection, mice received a single dose of the histamine H2R agonist dimaprit dihydrochloride (Tocris Bioscience) or a mock treatment with the vehicle. Dimaprit was administered intraperitoneally as 0.1 ml of dimaprit dihydrochloride (40 mg/ml) dissolved in sterile saline, and the vehicle (saline) was administered in an equivalent volume. One hour later, mice were challenged with S. Typhimurium for 4 hours and then assessed for gastric pH and intestinal S. Typhimurium burden.