5.2. Video-EEG recordings and analysis

Synchronized video-EEGs were recorded from 2.5 to 6.5-month-old WT and KI mice after electrode implantation (Pinnacle Technology, #8201) with a video-EEG monitoring system from Pinnacle Technology as described in (Arain et al., 2012). Briefly, mice were anesthetized with 1–3% isoflurane (vol/vol), the front edge of the head mount was placed 3.0–3.5 mm anterior of bregma and secured by four stainless steel screws and dental cement. Two EMG leads were inserted into the trapezius muscles. Mice recovered from implantation surgery for at least 1 week before EEG recording.

Video-EEG monitoring lasted for 24 h with freely moving mice in the chamber. The pre-amplifier filtered (1 Hz high pass) and amplified the signal (gain 100×), and additional filtering occurred at the data acquisition/conditioning system. The acquisition rate for EEG-EMG channels was 400 Hz and for the infrared camera was 15 frames/s. Video-EEG-EMG data were analyzed offline with Sirenia® Seizure software. The reviewer was blind to the genotypes.