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Modified seedbox screening test (MSST)

YL Yi Li
BC Boon Huat Cheah
YF Yu-Fu Fang
YK Yun-Hung Kuang
SL Shau-Ching Lin
CL Chung-Ta Liao
SH Shou-Horng Huang
YL Ya-Fen Lin
WC Wen-Po Chuang
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The MSST experimental setup was modified from the standard evaluation system of the IRRI [77]. Briefly, six rice varieties were planted in a square plastic box (1 m × 1 m). In each box, each variety had four hills with 3–5 seedlings. At the tillering stage (ca. 30–35 days after sowing), rice plants were infested with gravid female N. lugens adults at a density of 0.5–1 adults per plant. When the susceptible checks (TN1) were moderately wilted due to N. lugens infestation, the condition of each seedling was scored using the same standard evaluation system as that used in the SSST every 2 to 3 days. This MSST experiment was repeated three times.

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