2.2. Protein–protein interaction network construction and module analysis

XG Xin Gao
YL Yuan Liu
SZ Shaohui Zou
PL Pengqin Liu
JZ Jing Zhao
CY Changshun Yang
ML Mingxing Liang
JY Jinlian Yang
ask Ask a question
Favorite

After obtaining DEGs from the GSE157103 data set, we used the STRING database (https://string-db.org/) to construct a protein–protein interaction (PPI) network to understand the interaction between the DEGs. The MCODE plug‐in with default parameters in Cytoscape 3.7.1 was used for the PPI network module analysis. After obtaining the genes in each module, we used the DAVID database (https://david.ncifcrf.gov/) to perform Gene Ontology (GO) analysis of the module genes separately with a screening condition of p < 0.05 to understand the role of each module in COVID‐19.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A