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Firefly luciferase activity assay and cell viability
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The firefly luciferase activities of transfected EPCs were measured using a luciferase kit (Promega, Madison, WI, USA) and luminometer (Hidex Oy, Turku, Finland). For normalization of the luciferase activity to the number of remaining cells post-transfection in each well, a cell count was performed with a Coulter Counter (Beckman, Brea, CA, USA) 2 h, 24 h and 48 h after transfection. The final luciferase expression in each group is presented as light units/cell.
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