Wheat germ agglutinin (WGA) staining

HW Hai-Yan Wu
YZ Yi-Min Zhou
ZL Zhu-Qin Liao
JZ Jia-Wen Zhong
YL You-Bin Liu
HZ Hui Zhao
CL Chi-Qian Liang
RH Rui-Jin Huang
KP Kyu-Sang Park
SF Shan-Shan Feng
LZ Li Zheng
DC Dong-Qing Cai
XQ Xu-Feng Qi
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Sections were deparaffinized, rinsed three times in PBS, and fixed in 4% paraformaldehyde for 30 min at 37 °C. After washing three times in PBS, sections were then incubated for 10 min at room temperature with primary antibody against WGA conjugated to Alexa Fluor 488 (5 μg/ml, ThermoFisher, W11261). Sections were rinsed three time in PBS and mounted in Antifade Mounting Medium (Jackson ImmunoResearch Laboratories). To quantify the cell size, four independent hearts (9–13 sections) per group were captured with laser-scanning confocal microscope (LSM 700, Zeiss). Image-Pro Plus version 6.0 software was used to quantify the size of each cell.

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