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DPPH assay

VB Visarut Buranasudja
DR Dolly Rani
AM Ashwini Malla
KK Khwanlada Kobtrakul
SV Sornkanok Vimolmangkang
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The radical scavenging activities of tested extracts were evaluated by traditional DPPH assay18. Briefly, 100 µL of DPPH solution (0.008% w/v DPPH in methanol) was mixed with 100 µL of varying concentrations of callus extract in a 96-well microplate and incubated at 25 °C for 30 min. After incubation, the absorbance was recorded at 517 nm using CLARIOStar microplate reader (BMG Labtech; Ortenberg, Germany). 100% methanol was used as a control.

The percentage of radical scavenging activity (%RSA) of sample was calculated using the following formula:

OD517Control = Optical density at 517 nm of 100% methanol; OD517sample = Optical density at 517 nm of samples.

The concentration of extracts that resulted in 50% RSA was estimated from dose-response curve using GraphPad Prism version 9.0 (GraphPad Software).

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