The RSCU can be calculated by way of following formula: where xij shows the frequency of codon j coding for the ith amino acid and ni represented the number of synonymous codon encoding the ith amino acids. The interpretation for the RSCU is that RSCU greater than one indicates more frequency while RSCU small than 1 indicates less frequency in a gene for the particular codon. Whereas RSCU > 1.6 indicates over represented codon for the corresponding amino acid (Wong et al., 2010).
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