2.4.2. Agarose gel electrophoresis of DNA extraction

RT Rebecca Tshabalala
AK Adia Kabelinde
CT Christ-DONALD KAPTCHOUANG TCHATCHOUANG
CA Collins Njie Ateba
MM Madira Coutlyne Manganyi
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The presence of DNA extracts was confirmed by electrophoresis on a 1% (w/v) agarose gel in 1X TAE buffer. The gel separation was done at 80 V for 45 min according to protocol and the electrophoretic pattern was analysed on a UV illuminator (Bio-Rad Laboratories, USA) in the Molecular Microbiology laboratory at the North-West University Mafikeng.

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