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RNA was isolated from tissues using peqGOLD TriFast, by homogenizing tissues with a TissueLyser (QIAGEN) and purifying RNA by NucleoSpin RNAII Kit. Afterward, cDNA was prepared with High-Capacity cDNA Archive Kit. Gene expression was assessed using Taqman assays supplied as assays-on-demands, and data were normalized to the gene expression levels of the housekeeper Tbp.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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