Tissue Processing

Mice were euthanized with an overdose of sodium pentobarbital (100 mg/kg, Glostrup Hospital) and perfused with ice-cold PBS. For reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), brains and spinal cords were placed in 0.5 ml TriZol Reagent (Ambion) and stored at −80°C until RNA extraction. For flow cytometry, CNS tissue was placed in ice-cold PBS. For histology, brains and spinal cords were post-fixed with 4% paraformaldehyde (PFA), immersed in 30% sucrose in PBS, then frozen embedded in Killik cryostat embedding medium (Bio-Optica, Milano, Italy) and 16 μm thick tissue sections were cut on a cryostat (Leica).