Epitope mapping

To identify epitopes recognised by MEH63, MEH158 and MEH183, a Ph.D.TM‐C7C Phage Display Peptide Library (New England Biolabs, Beverly, MA, USA) was screened against the scFv (MEH63, MEH158 or MEH183), according to the manufacturer’s instructions. Single‐stranded DNAs of 12 phage clones (four phage clones from the third round of biopanning on MEH63, MEH158 or MEH183) were extracted and sequenced, according to the Ph.D.‐C7C Kit instructions. The inference of amino acid sequences was analysed in Gene Runner version 6.0 and checked in the Biopanning Data Bank to remove probable target‐unrelated peptides (MimoDB) (http://immunet.cn/bdb/). 108 , 109 Next, the selected peptides were blasted against the NCBI protein database for S. aureus , S. epidermidis and S. pyogenes , and proteins with scores above 18 were selected as the candidate proteins. 108 , 109