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Genomic DNA was extracted from MIN6 cells treatment with 0.5 mmol/l palmitate or extracted from the islets of db/db or control mice (n = 7). The DNA was subjected to sodium bisulphite modification using EpiTect Bisulfite Kits (QIAGEN) following the manufacturer’s protocols. Sodium bisulphite-treated DNA was PCR amplified using primers (Table S4) predicted on MethPrimer website and EpiTaq™HS (Takara). Amplified bisulfate PCR products in each group were cloned into the T-Vector pMD19 (Takara), and over 20 clones were sequenced.
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