Transcriptomic analysis

Two biological replicates of RNA samples were isolated from A549 cells transduced with pMSCV-dnCRTC or pMSCV-GFP retroviruses at 72 hr post-infection and were then subjected to microarray experiment using GeneChip Human Transcriptome Array 2.0 (Affymetrix) at the Genomics Core at Sanford Burnham Research Institute. The same samples were also subjected to RNAseq analysis by Novogene. In brief, RNA-seq libraries (non-strand-specific, paired end) were prepared with the NEBNext Ultra RNA Library Prep Kit (Illumina) and were sequenced according to the paired-end 150 bp protocol using NovaSeq 6000. The data were analyzed as previously described (Yang et al., 2019; Chen et al., 2016; Chen et al., 2015). Genes with an absolute fold change ≥2 and an FDR p-value<0.5 were considered as significantly differentially expressed.