Smek1-floxed mice were generated by delivering a linearized vector to ES cells (C57BL/6) by Cyagen Biosciences. Smek1fl/+ Sox2-Cre and Smek1fl/fl Sox2-Cre mice are referred to as Smek1-/+ and Smek1-/- mice, respectively. Sox2-Cre mice were from Shanghai Model Organisms Center. Mice were group housed under a 12 h light/dark cycle (light between 07:00 and 19:00) in a temperature-controlled room. All mice were bred and housed under specific pathogen-free conditions at Shandong University.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.