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2.9. Biochemical Assays and Enzyme-Linked Immunosorbent Assay

XY Xiao-Chen Yu
ZL Zhen Li
XL Xin-Ran Liu
JH Jia-Ni Hu
RL Rui Liu
NZ Na Zhu
YL Yong Li
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At the end of the experiment, the mice were killed by eyeball blood sampling and cervical dislocation. After the animals were killed, the liver and brain were separated on ice immediately, and the excess tissue was removed for standby. The levels of serum, liver, and brain oxidative stress biomarkers (SOD, GSH-Px, MDA, PC) were determined by assay kits, according to the protocol provided by the manufacturer. The brain tissue homogenate of different groups was used to estimate the inflammatory parameters (TNF-α, IL-1β), cholinergic system related enzymes (AChE, ChAT) by assay, according to the protocol provided by the manufacturer.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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