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2.6. Custom Cloning into a pET32a Expression System
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The best clone identified in the immunoscreening and sequence analysis was selected to produce a recombinant protein. The nucleotide sequence of the selected clone was sent to a scientific company (EPOCH Life Science Inc., Missouri City, TX, USA) for codon optimization, nucleotide synthesis, and cloning into the pET32a expression system.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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