4.3. Determination of Minimum Inhibitory Concentrations

MIC values were determined by the broth microdilution method and the significance of the values obtained was interpreted following the guidelines of the Clinical and Laboratory Standards for Antimicrobial Susceptibility Testing. [20]. The effect of human serum on the antimicrobial effect of the molecules tested was determined in a similar way to the MIC method used above in a solution of 50% human serum plus Mueller–Hinton broth.