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Akt1/2 shRNA lentiviral particles (sc-37030-V) were provided by Santa Cruz Biotech and were added directly to primary NSCLC cells. After 24 h, puromycin (5.0 μg/ml) was added to select stable cells, where over 95% Akt1/2 protein knockdown efficiency was achieved.
Copyright and License information: ©2021 Zha, Xia, Ye, Hu, Zhang, Xiao, Yu, Xu and Xu
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