Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Western blot analysis

YZ Yi Zhao
JY Juan Yang
JL Jiarui Liu
YC Yiqing Cai
YH Yang Han
SH Shunfeng Hu
SR Shuai Ren
XZ Xiangxiang Zhou
XW Xin Wang
ask Ask a question
Favorite

Western blot analysis was carried out as previously described [30]. Primary antibodies used in western blot were listed as below: PLK4 (#NBP1-33042, Novus, USA), LATS1 (#3477, Cell Signaling Technology, USA), phospho-LATS1 (Thr1079) (#8654, Cell Signaling Technology), YAP (#14074, Cell Signaling Technology), phospho-YAP (Ser127) (#13308, Cell Signaling Technology), cleaved PARP (#5625, Cell Signaling Technology), γ-H2AX (Ser139) (#9718, Cell Signaling Technology), phospho-ATM (Ser1981) (#5883, Cell Signaling Technology), phospho-ATR (Ser428) (#2853, Cell Signaling Technology), phospho-Chk1(Ser345) (#2348, Cell Signaling Technology), phospho-Chk2 (Thr68) (#2197, Cell Signaling Technology), anti-Histone H3 (#4499, Cell Signaling Technology). β-actin was used as a loading control. Chemiluminescent signals were detected using the Amersham Imager 600 imaging system (General Electric, USA). ImageJ software (National Institutes of Health, Bethesda, MD, USA) was used to quantify the protein bands normalized to control.

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A