Cell transfection with lentivirus, lncRNA smart silencer, miRNA inhibitor, miRNA mimics siRNA and plasmid

XS Xiaonan Sheng
HD Huijuan Dai
YD Yueyao Du
JP Jing Peng
RS Rui Sha
FY Fan Yang
LZ Liheng Zhou
YL Yanping Lin
SX Shuguang Xu
YW Yifan Wu
WY Wenjin Yin
JL Jinsong Lu
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Cells were transfected with lentivirus vectors overexpressing CARMN or control ones (purchased from GenePharma Shanghai, China) for stably overexpressing CARMN. CARMN smart silencer [8] was bought from Ribobio (Guangzhou, China).

MiR143-3p inhibitor and inhibitor control (GenePharma, Shanghai, China), miR143-3p mimics (purchased from Genomeditech, China), siRNA of DROSHA/DICER1/MCM5 (purchased from Genomeditech, China), plasmid overexpressing exon5 of CARMN (purchased from Genechem, Shanghai, China), whose sequence are all listed in Supplementary Table 2, were transfected with Polyplus Transfection jetPRIME (France). After 48 h, the cells were collected for detecting efficiency and further use.

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