Cells were transfected with lentivirus vectors overexpressing CARMN or control ones (purchased from GenePharma Shanghai, China) for stably overexpressing CARMN. CARMN smart silencer [8] was bought from Ribobio (Guangzhou, China).
MiR143-3p inhibitor and inhibitor control (GenePharma, Shanghai, China), miR143-3p mimics (purchased from Genomeditech, China), siRNA of DROSHA/DICER1/MCM5 (purchased from Genomeditech, China), plasmid overexpressing exon5 of CARMN (purchased from Genechem, Shanghai, China), whose sequence are all listed in Supplementary Table 2, were transfected with Polyplus Transfection jetPRIME (France). After 48 h, the cells were collected for detecting efficiency and further use.
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