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Preparation and infection of lentivirus vectors
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The lentivirus vector expressing full-length mouse CRT under the control of the human eukaryotic translation elongation factor 1 α1 promoter and the lentivirus vector alone was purchased from VectorBuilder (Chicago, IL, USA). Viral stocks had titers of ~ 109 plaque-forming units/ml. Hippocampal neurons were infected with the CRT-expressing (LV-CRT) or control (LV-control) lentivirus vector at a multiplicity of infection 10 for 16 h and further incubated for 48–72 h.
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