Analysis of Tandem Duplication Event

YL Yajun Liang
JW Junduo Wang
JZ Juyun Zheng
ZG Zhaolong Gong
ZL Zhiqiang Li
XA Xiantao Ai
XL Xueyuan Li
QC Quanjia Chen
request Request a Protocol
ask Ask a question
Favorite

Following the identification of tandemly duplicated genes in PTGBase, all-against-all BLAST of protein sequences in 13 cotton species was employed to identify the paralogous gene pairs within single cotton species with E-value cut-off ≤1e-20 (Yu et al., 2015). After confirmation of paralogous gene pairs with high similarity anchored on closer location of identical genomic regions, tandemly duplicated genes were obtained in different cotton species, and one unrelated gene was allowed within one tandem array.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A