Following the identification of tandemly duplicated genes in PTGBase, all-against-all BLAST of protein sequences in 13 cotton species was employed to identify the paralogous gene pairs within single cotton species with E-value cut-off ≤1e-20 (Yu et al., 2015). After confirmation of paralogous gene pairs with high similarity anchored on closer location of identical genomic regions, tandemly duplicated genes were obtained in different cotton species, and one unrelated gene was allowed within one tandem array.
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