Plasmatic levels of uric acid were determined by a colorimetric method (Barham and Trinder, 1972) based on the reduction of uric acid by the uricase enzyme. In this reaction, hydrogen peroxide is released and forms a chromogenic compound evaluated spectrophotometrically at 550 nm.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.