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Isolation of Extracellular Vesicles by Tangential Flow Filtration
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After removing cells and debris by centrifugation at 500 × g (5 min) and 2,000 × g (10 min) as described above, the EV containing supernatant was filtered (0.45 μm) and transferred to TFF-Easy 20-nm pores (HansaBioMed/Lonza, Tallinn, Estonia) for EV concentration. The EV concentration process was described in the manufacturer’s protocol (HansaBioMed/Lonza), and EVs were finally diafiltrated in RNase-free PBS.
Copyright and License information: ©2021 Phan, Divakarla, Yeo, Lei, Tharkar, Pansani, Leslie, Tong, Coleman, Jämting, Du Plessis, New, Kalionis, Demokritou, Woo, Cho and Chrzanowski.
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