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LUHMES cells were differentiated in 96-well poly-D-lysine-coated plates (Greiner, catalog no. 655946). Then, the cells were incubated 30 min with 50 nM MitoTracker Green (ThermoFisher Scientific, catalog no. M7514) and Hoechst 33342 (Molecular Probes, H3570). Images were taken on the LSM800 confocal microscope (Zeiss) with a 40× objective and, before imaging, the medium was replaced by HBSS−/− (GIBCO, 14175-053).
Copyright and License information: ©2021 2021. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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