The MaxCyte® or FectoPRO® transfection cell culture medium was centrifuged and filtered through a 0.22 µm filter to remove cells and debris, then loaded onto a HiTrap™ MabSelect SuRe™ column on the AKTA Pure system pre-equilibrated with 10 mM Na Phosphate and 150 mM NaCl at pH 7.0. After loading, the column was washed with ten column volumes of the same buffer. The protein was eluted with 100 mM sodium acetate, pH 3.6, then immediately neutralized using 2 M Tris pH 8.0. The elution fractions were pooled and dialyzed into 10 mM HEPES and 150 mM sodium chloride at pH 7.4.
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