Sample size, replication, and sample allocation

Sample sizes, as indicated in figure legends, were determined based on the observed variability across measurements. All experiments have been replicated in multiple independent trials/animals (typically >3) with the exception that only one round of NGS was carried out. Cells and animals were randomly allocated to each experimental groups. All groups to be compared were carried out side-by-side. The researchers were not blinded to group allocation during data collection and analysis. However, the FOVs were selected either predetermined or in the absence of knowledge of the color channel under investigation.