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IC50 calculation

XH Xuan Huang
KR Kasper C.D. Roet
LZ Liying Zhang
AB Amy Brault
AB Allison P. Berg
AJ Anne B. Jefferson
JK Jackie Klug-McLeod
KL Karen L. Leach
FV Fabien Vincent
HY Hongying Yang
AC Anthony J. Coyle
LJ Lyn H. Jones
DF Devlin Frost
OW Ole Wiskow
KC Kuchuan Chen
RM Rie Maeda
AG Alyssa Grantham
MD Mary K. Dornon
JK Joseph R. Klim
MS Marco T. Siekmann
DZ Dongyi Zhao
SL Seungkyu Lee
KE Kevin Eggan
CW Clifford J. Woolf
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The mean and standard deviation of the HPE (100% effect) and ZPE (0% effect) were calculated for each plate. The compound data at each concentration was converted into % effect, using the ZPE and HPE controls as 0% and 100% activity, respectively. The formula used for converting each well reading into % effect is: (Well activity value – ZPE activity value) × 100/(HPE activity value-ZPE activity value). Then the % effect was plotted against log10 [compound]. An unconstrained sigmoid curve was thus fitted using a 4 parameter logistic model, and an IC50 was reported as the midpoint of the curve.

Copyright and License information:  ©2021
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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