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BA Bokyung Ahn
YC Yang Seok Chae
SL Soo Kyung Lee
MK Moa Kim
HK Hyeon Soo Kim
JM Ji Wook Moon
SP Sun-Hwa Park
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One hundred and five CRC and adjacent normal tissue samples and eleven normal colon tissue samples were obtained from the Department of Colorectal Surgery, Korea University Medical Center. Normal tissue samples were collected through laparoscopic surgery with consent from the hernia patient. The tissues for the epigenetic study were used after obtaining approval from the Institutional Review Board of Korea University College of Medicine (IRB No. KU-IRB-13-84-A-1) and informed consent was obtained from all subjects or, if subjects are under 18, from a parent and/or legal guardian. Every fresh tissue sample was frozen in liquid nitrogen after resection and stored in a refrigerator at − 80 °C. Additionally, 676 surgically resected CRC tissues were retrieved from the Department of Pathology, Korea University Anam hospital, from January 2007 to December 2010. Patients who received preoperative treatment and cases with no available block were excluded, and a total of 615 CRC cases, 26 cases of metastases to the liver and lungs, and 25 cases of adjacent normal colon in patients with metastasis were reviewed. All haematoxylin and eosin (H&E) slides of the cases were reviewed by two pathologists (BKA and YSC), and the most representative area was retrieved for tissue microarray. Clinicopathologic data, including age, sex, survival months, survival, tumour location, tumour size, grade, pathologic T (pT) category, pathologic N (pN) stage, lymphovascular invasion (LVI), perineural invasion (PNI), lymph node (LN) status, distant metastasis, and resection marginal status were reviewed. The TNM stages were adjusted to the 8th American Joint Committee on Cancer Staging Manual. All experimental protocols were approved by the Institutional Review Boards of Korea University Anam Hospital (K2018-2161-001). All methods were carried out in accordance with relevant guidelines and regulations. The characteristics of each subject are summarized in Table ​Table33.

Clinicopathologic characteristics of colorectal cancer patients.

AJCC stage American Joint Committee on Cancer stage, RM resection margin.

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