2.5. Effects of saponin 0.0125% on human tissue lysis

To test whether saponin 0.0125% was able to induce human cell lysis, resected human colon biopsies of an estimated size of 5 mm were processed according to our optimized protocol up to the step of selective cell lysis using saponin (Figure ​(Figure11 and Appendix 3). During this last step, cell pellets were incubated with either 0.0125% saponin or PBS in turboDNAse buffer, but without turboDNAse enzyme. Samples were incubated at 37°C for 30 min to lyse the cells, and the supernatant was cleared from cell debris by two centrifugation cycles of 10 min at 10,000×g at 4°C. DNA in the supernatant was precipitated with 100% ethanol and centrifuged at 10,000×g at 4°C for 20 min. Precipitated DNA was washed with 70% ethanol and centrifuged at 10,000×g at 4°C for 20 min. Lastly, DNA was air‐dried and resuspended dH₂O.