Cells were seeded in 96-well plates at a density of 2 × 103 cells per well in the corresponding medium. Cells were assessed by the CCK8 (APExBio) assay after 0, 24, 48, or 72 h of incubation. The absorbance of each well at 450 nm was measured. Population doubling time (PDT) was calculated using the formula PDT = .
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