Cell Counting Kit (CCK)-8 Cell Growth Assay

ZL Zhaoqing Li
WZ Wenying Zhuo
LC Lini Chen
XZ Xun Zhang
CC Cong Chen
DH Dengdi Hu
YC Yongxia Chen
JY Jingjing Yang
YZ Yulu Zhou
MM Misha Mao
LX Ling Xu
SJ Siwei Ju
JS Jun Shen
QW Qinchuan Wang
MD Minjun Dong
SX Shuduo Xie
JZ Jichun Zhou
LW Linbo Wang
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Cells were seeded in 96-well plates at a density of 2 × 103 cells per well in the corresponding medium. Cells were assessed by the CCK8 (APExBio) assay after 0, 24, 48, or 72 h of incubation. The absorbance of each well at 450 nm was measured. Population doubling time (PDT) was calculated using the formula PDT = 0.693t/ln(NtN0).

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