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The enzyme activity was calculated according to methodology given by Kono [77] with slight modifications. 0.05 ml sample, 1.5 ml sodium carbonate buffer, 0.5 ml NBT (Nitroblue tetrazolium), 0.1 ml TritonX-100, 0.1 ml hydroxylamine hydrochloride were taken in cuvette and increase in absorbance was recorded at 540 nm. The enzyme activity was expressed as μM/ml (haemolymph).
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