Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Materials

KS Kai Song
JS Jingjing Sun
WW Wei Wang
JH Jianhua Hao
request Request a Protocol
ask Ask a question
Favorite

my20 gene (GenBank accession no. MW561626) originated from the metagenome sequencing of marine microorganisms in the Mariana Trench. The gene my20 was codon optimized, synthesized, and cloned into pET-24a(+) by Genewiz (Wuhan, China). Escherichia coli BL21(DE3) was used for heterologous expression. Luria–Bertani (LB) medium was used to cultivate E. coli strains. AA-2G was obtained from DB (Shanghai, China). Ni2+-NTA HP resin was purchased from General Electric Company (United States). Other reagents were obtained from Shanghai Sinopharm Co., Ltd. (Shanghai, China).

Copyright and License information:  ©2021 Song, Sun, Wang and Hao.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A