Crystal Violet Staining

ZB Zhaoshi Bai
QZ Qing Zhou
HZ Huayun Zhu
XY Xinyue Ye
PW Pingping Wu
LM Lingman Ma
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Cells were seeded into six-well plates at a density of 5000 cells/well and treated with different concentrations of QTMP (5~20 μg/ml) for 48 h. Cells were shifted into drug-free fresh medium for two weeks and were then fixed with 4% paraformaldehyde, stained with crystal violet for 40 min and photographed with a digital camera (26, 27).

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