Intra-peritoneal glucose tolerance test (IPGTT)

The glucose tolerance test (IMPC_IPG_001) was used to measure the clearance of intraperitoneally injected glucose via adipose tissue. Mice were fasted for ~18 h prior to the procedure. Control blood glucose blood samples were taken by alcohol cleaning and then scoring the tip of the tail with a sterile scalpel blade and milking a small (5 µL) sample onto a glucose test strip. The bleeding was controlled through the application of dry gauze and pressure hemostasis. Following the initial blood sampling, 20% glucose solution was injected intraperitoneally at a dose of 2 g/kg (µL volume injected = 10 × body weight in grams; average mouse 0.25–0.3 mL). Blood glucose measurements were then taken at 15, 30, 60, and 120 min after glucose injection by agitation and removal of the previous tail tip clot and milking of ~5 µL of blood onto the test strip. Total blood collected for the procedure was 25 µL per mouse.