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After 6 days of osteoblast differentiation, cells were fixed with acetone/10 mM citrate buffer pH4.2 (1.5:1 ratio) at room temperature for 5 min and incubated for 1 h at room temperature with ALP substrate staining solution containing 0.2 mg/ml Naphtol-AS-TR-phosphate dissolved in distilled water (1:5) and 0.417 mg/ml Fast Red dissolved in 0.1 M Tris buffer.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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