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Gene set enrichment analysis

TZ Tao Zhu
JZ Juyan Zheng
WZ Wei Zhuo
PP Pinhua Pan
ML Min Li
WZ Wei Zhang
HZ Honghao Zhou
YG Yang Gao
XL Xi Li
ZL Zhaoqian Liu
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Gene set enrichment analysis (GSEA) to explore ETV4-associated molecular functions in breast cancer tissues was carried out by using TCGA RNA-seq data of 1104 breast cancer samples downloaded from UCSC Xena (https://xena.ucsc.edu/). The patients were ranked according to ETV4 mRNA expression and divided into 2 groups based on the median ETV4 expression. Pre-defined gene sets were acquired from the Molecular Signatures Database (MSigDB) and published studies42,69. Gene sets with a false discovery rate (FDR) less than 0.25 were considered significant, as previously reported69,70.

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