AD core toxicity in M17 neuroblastoma cells

M17 cells were treated with different concentrations of recombinant AD core polymerized by shaking for 24 hours in the absence of inducer. 72 hours following treatment, media was collected for an LDH assay following manufacturer’s protocols (Promega). Cells were washed with PBS, treated with trypsin for 2 minutes, and collected by ultracentrifugation. Cell pellets were washed in PBS, and subsequently lysed in cell lysis buffer (50 mM Tris, 274 mM NaCl, 5 mM KCl, 5mM EDTA, 1% Triton X-100, 1% SDS, 1 mM PMSF, and protease and phosphatase inhibitor cocktails).