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Intraperitoneal Glucose Tolerance Test (IPGTT) and Intraperitoneal Insulin Tolerance Test (IPITT)
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An IPGTT was performed on conscious animals after a 12-h fast followed by an i.p. injection of glucose at 2 g/kg body mass (2 g kg−1). An IPITT was performed on mice after a 4-h fast followed by an i.p. injection of 0.75 units of human regular insulin per kg body mass (0.75 µ kg−1). Blood glucose levels were obtained from tail vein blood 0, 15, 30, 60, 90, and 120 min after injection.
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